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. 2021 Sep 3;12:720354. doi: 10.3389/fimmu.2021.720354

Figure 7.

Figure 7

The in vitro inhibition experiment of mucosa-associated invariant T (MAIT) cells on CD4+ T cells and immunofluorescence experiment of intestinal tissue. (A) MAIT cells and CD4+ T cells were isolated from the peripheral blood of four healthy donors. Here, 5,6-carboxy-fluorescein diacetate succinimidyl ester (CFSE)-labeled CD4+ T cells with CD3/CD28 bead stimulation and MAIT cells or CD4+ T cells were mixed and cultured at a ratio of 2:1, 1:2, 1:4, and 1:8 for 4 days. Representative flow cytometric analysis of CFSE dilutions in CD4+ T cells. (B) Immunofluorescence of intestinal tissues of healthy people, gut aGVHD lesion sites and peri-gut aGVHD sites of gut aGVHD patients. (C) Comparison of CD161 staining intensity, CD161-positive cell ratio, number, and density between healthy person intestinal tissue (n = 2) and intestinal lesion tissue of gut aGVHD patients (n = 5). Since only one case of gut aGVHD patient in the experiment received the intestinal tissue of peri-gut aGVHD site, there is no peri-gut aGVHD group in the comparison. (D) Comparison of CD8+CD161+ cell ratio, number, and density between healthy person intestinal tissue (n = 2) and intestinal lesion tissue of gut aGVHD patients (n = 5).