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. 2021 Feb 2;224(6):1060–1068. doi: 10.1093/infdis/jiab054

Table 4.

Detection of Zika Virus Neutralizing Antibodies According to Diagnosis of Zika Infection

Laboratory Diagnosis of Zika Infectionc
No. Positived
ZIKV IgM Only ZIKV IgM + ZIKV RNA ZIKV RNA Only No. Negative No. Not Done
Age, moa VNTb + VNT − VNT + VNT − VNT + VNT − VNT + VNT − VNT + VNT −
0–3 7 12 1 8 2 9 1 1
3–6 4 2 5 7 2 9 1 1
6–12 1 3 3 2 1 3
> 12 1 4 4 4
Total 11 3 17 1 16 11 21 8 1 9

Abbreviations: IgM, immunoglobulin M; VNT, virus neutralization test; ZIKV, Zika virus.

aAge of infants at sample collection to carry out ZIKV VNT.

bVNT was considered positive in samples with ZIKV neutralizing antibodies titer >1:40.

cLaboratory diagnosis of Zika infection was performed by detection of ZIKV IgM and ZIKV RNA in blood and/or urine. Investigation was positive in 59 children, negative in 29, and not done in 10 infants.

dDistribution analysis of ZIKV VNT results was performed between infants with positive results for only ZIKV IgM, ZIKV IgM and ZIKV RNA, and only ZIKV RNA. χ 2 test, P = .027.