Figure 1.

Schematic of vaccine constructs. We utilized the attenuated rabies vaccine strain SAD B19 as the backbone to generate our recombinant vaccines. The rabies genome encodes for 5 genes: N (nucleoprotein), P (phosphoprotein), M (matrix protein), G (glycoprotein), and L (RNA-dependent RNA polymerase). We previously introduced a trancription cassette with restriction sites BsiWi and NheI between N and P genes of the SAD B19 vaccine vector. The 333 (arginine to glutamic acid; shown as ★) mutation in the rabies glycoprotein (G) further reduces its neurotropism. In this study we used the chemically inactivated rabies-vectored Ebola (FILORAB1), Sudan (FILORAB2), Marburg (FILORAB3), and Lassa (LASSARAB) virus vaccines that express the viral hemorrhagic fever GPs inserted between the N and P genes of the rabies vaccine vector.