Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | C57BL/6J | The Jackson Laboratory | Stock no: 000664 | |
| Genetic reagent (M. musculus) | Trpv4conditional knockout in EC | Dr. Swapnil SonkusarePMID:32008372 | ||
| Genetic reagent (M. musculus) | Trpv4 conditional knockout in SMC | Dr. Swapnil SonkusarePMID:33879616 | ||
| Genetic reagent (M. musculus) | Panx1 conditional knockout in EC | Dr. Brant IsaksonPMID:26242575 | ||
| Genetic reagent (M. musculus) | Panx1 conditional knockout in SMC | Dr. Brant IsaksonPMID:25690012 | ||
| Genetic reagent (M. musculus) | Cav1 conditional knockout in EC | Dr. Swapnil SonkusarePMID:33879616Dr. Richard MinshallPMID:22323292 | ||
| Genetic reagent (M. musculus) | P2ry2fl/fl mice | Dr. Cheikh SeyePMID:27856454 | ||
| Genetic reagent (M. musculus) | Cdh5-Optoα1AR- IRES-lacZ |
CHROMus (Cornell University, USA) |
||
| Antibody | TRPV4 antibody (aa100-150), (mouse polyclonal) | LifeSpan Bioscience Inc | Cat. #: LS-C94498;RRID:AB_2893149 | (1:200) |
| Antibody | Anti-caveolin-1 antibody - caveolae marker (rabbit polyclonal) | Abcam plc | Cat. #: Ab2910;RRID:AB_303405 | (1:500) |
| Antibody | Caveolin-1 antibody (7C8) (mouse monoclonal) | Novus Biologicals, LLC | Cat. #: NB100-615;RRID:AB_10003431 | (1:200) |
| Antibody | PKC (mouse monoclonal) | Santa Cruz Biotechnology, Inc |
Cat. #: SC-17769;RRID:AB_628139 | (1:250) |
| Antibody | Panx1 (rabbit polyclonal) | Alomone Labs | Cat. #: ACC-234;RRID:AB_2340917 | (1:100) |
| Antibody | P2Y2R (rabbit polyclonal) | Alomone Labs | Cat. #: APR-010;RRID:AB_2040078 | (1:250) |
| Antibody | P2Y1R (rabbit polyclonal) | Alomone Labs | Cat. #: APR-009;RRID:AB_2040070 | (1:100) |
| Chemical compound, drug | GSK2193874 | Tocris Bioscience | Cat. #: 5106/5 | |
| Chemical compound, drug | Cyclopiazonic acid (CPA) | Tocris Bioscience | Cat. #: 1235/10 | |
| Chemical compound, drug | GSK1016790A | Tocris Bioscience | Cat. #: 6433/10 | |
| Chemical compound, drug | Phorbol 12-myristate 13-acetate (PMA) | Tocris Bioscience | Cat. #: 1201/1 | |
| Chemical compound, drug | AR-C 118925XX | Tocris Bioscience | Cat. #: 4890/5 | |
| Chemical compound, drug | 2-Thio UTP tetrasodium salt | Tocris Bioscience | Cat. #: 3280/1 | |
| Chemical compound, drug | MRS2179 | Tocris Bioscience | Cat. #: 0900/10 | |
| Chemical compound, drug | U-73122 | Tocris Bioscience | Cat. #: 1268/10 | |
| Chemical compound, drug | NS309 | Tocris Bioscience | Cat. #: 3895/10 | |
| Chemical compound, drug | ARL-67156 | Tocris Bioscience | Cat. #: 1283/10 | |
| Other | Fluo-4-AM | Invitrogen | Cat. #: F14201 | |
| Chemical compound, drug | 1-O-9Z-octadecenoyl-2-O-acetyl-sn-glycerol (OAG) | Cayman Chemicals | Cat. #: 62600 | |
| Chemical compound, drug | PPADS | Cayman Chemicals | Cat. #: 14537 | |
| Chemical compound, drug | Gö-6976 | Cayman Chemicals | Cat. #: 13310 | |
| Chemical compound, drug | JNJ-47965567 | Cayman Chemicals | Cat. #: 21895 | |
| Chemical compound, drug | U46619 | Cayman Chemicals | Cat. #: 16452 | |
| Chemical compound, drug | Tamoxifen | Sigma-Aldrich | Cat. #: T5648 | |
| Peptide, recombinant protein | Apyrase | Sigma-Aldrich | Cat. #: A6535 | |
| Software, algorithm | LabChart8 | ADInstruments https://www.adinstruments.com/products/labchart |
RRID:SCR_017551 | |
| Software, algorithm | Segment version 2.0 R5292 | Twilio(http://segment.heiberg.se) | ||
| Software, algorithm | IonOptix | IonOptix, LLC ( https://www.ionoptix.com/products/software/ionwizard-core-and-analysis/) |
||
| Software, algorithm | SparkAn | Dr. Adrian Bonev, University of Vermont, Burlington, VT, USA PMID:22556255 |
||
| Software, algorithm | ClampFit10.3 | Molecular Devices (https://www.moleculardevices.com/) |
RRID:SCR_011323 | |
| Software, algorithm | ImageJ | National Institutes of Health (https://imagej.nih.gov/ij/) |
RRID:SCR_003070 | |
| Software, algorithm | PatchMaster v2x90 program | Harvard Bioscience https://www.harvardbioscience.com/ |
RRID:SCR_000034 | |
| Software, algorithm | FitMaster v2x73.2 | Harvard Bioscience https://www.harvardbioscience.com/ |
RRID:SCR_016233 | |
| Software, algorithm | MATLAB R2018a | MathWorks https://www.mathworks.com/products/matlab.html |
RRID:SCR_013499 | |
| Software, algorithm | CorelDraw Graphics Suite X7 | CorelDraw (https://www.coreldraw.com/en) |
RRID:SCR_014235 | |
| Software, algorithm | GraphPad Prism 8.3.0 | GraphPad Software, Inc (https://www.graphpad.com/) |
RRID:SCR_002798 | |
| Software, algorithm | GLIMMPSE software |
(https://glimmpse.samplesizeshop.org/) |
RRID:SCR_016297 | |
| Software, algorithm | Biorender | http://biorender.com | RRID:SCR_018361 |