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. 2021 Sep 2;10:e69438. doi: 10.7554/eLife.69438

Figure 4. Reducing cytochrome C oxidase prevents trauma-induced ROS production.

(A) Time-course of peroxide levels in C. elegans post-trauma. n=three repeats per group. (B) GFP retention in dopaminergic neurons of C. elegans ectopically expressing the mitochondrial-targeted KillerRed fluorophore in neurons. n=three repeats per group. (C) Oxygen consumption rate, OCR, in Surf1+/+ and Surf1-/- mouse embryonic fibroblasts. n=3 Surf1+/+, n=2 Surf1-/-. (D) Representative micrographs of TOM20 (green) and DAPI (blue) in Surf1+/+ and Surf1-/-mouse embryonic fibroblasts. Scale bar=20 μm. (E) Trauma-induced superoxide production in mitochondria from Surf1+/+ and Surf1-/- brain single cell suspensions 2 hr post-injury. n=three per group. (F) Oxidized glutathione (GSSG) over glutathione (GSH) from metabolite analysis of Surf1+/+ and Surf1-/- brain tissue subject to concussive injury. n=three per group. Data are mean ± SEM. *p ≤ 0.0116, ns (not significant, p = 0.0524) and ****p ≤ 0.0001.

Figure 4.

Figure 4—figure supplement 1. Mitochondrial dynamics in embryonic fibroblasts.

Figure 4—figure supplement 1.

(A) Mitochondrial membrane potential by tetramethylrhodamine, ethyl ester (TMRE) fluorescence. n=3 Surf1+/+, n=3 Surf1-/-. (B) Percent fibroblasts with toroidal mitochondrial morphology. n=10 Surf1+/+, n=19 Surf1-/- micrographs. (C) Basal mitochondrial superoxide levels from Surf1+/+ and Surf1-/- brains. n=3 per group. Data are mean ± SEM. *p ≤ 0.05 and ***p ≤ 0.001.