Skip to main content
. 2021 Aug 16;10:e62001. doi: 10.7554/eLife.62001

Figure 2. dI2 neurons project to the cerebellum.

(A) Experimental setup for labeling dI2 neurons that project to the cerebellum. dI2 neurons were genetically targeted at HH18, and precerebellar neurons were labeled using intracerebellar injection of replication-defective HSV-LacZ or PRV-Cherry at embryonic day (E) 15. The abbreviations in the coordinates: R: rostral; C: caudal. (B) A cross-section of E17 brainstem and cerebellum. The dashed polygon in (B′) is magnified in (B). dI2 axons reach the cerebellum, enter into it via the superior cerebellar peduncle, and cross the cerebellar midline. Calbindin (Purkinje neurons, magenta [B′] or red [B]). Abbreviations in the coordinates: D: dorsal; V: ventral. (C) A cross-section of E17 cerebellar cortex. Lumbar-originating dI2 synapses (cyan) in the granular layer of the anterior cerebellar cortex. Calbindin (Purkinje neurons, magenta), synaptotagmin (yellow). (D) A cross-section of an E15 embryo at the lumbar spinal cord level (sciatic plexus level). Precerebellar neurons were infected and labeled with HSV-LacZ (magenta), and dI2 neurons expressed GFP (cyan). A large-diameter dI2 neuron coexpressing LacZ and GFP is indicated by an arrow (magnification of the two channels in the insets). (E) Density plots of dI2 and precerebellar neurons (density values 10–90%) in the sciatic plexus segments (N = 374 and N = 289 cells, respectively). (F) PRV-Cherry-labeled precerebellar neurons (magenta) are in contact with dI2 axonal terminals (cyan). (G) Density plots of dI2 synapses and precerebellar neuron somata (density values 10–90%) in the sciatic plexus segments (N = 4735 synapses and N = 289 cells, respectively). (H) The laminar distribution of precerebellar neurons, dI2 neurons, and dI2 synapses at the sciatic level. See Figure 2—source data 1.

Figure 2—source data 1. Localization of precerebellar neurons and dI2 synapses at the sciatic level.
The X/Y coordinates of precerebellar neurons (Figure 2E) and dI2 synapses (Figure 2G) at the sciatic level.

Figure 2.

Figure 2—figure supplement 1. Cerebellar and central cerebellar nucleus targets of dI2 neurons.

Figure 2—figure supplement 1.

(A) dI2 synapses in the central cerebellar nuclei. A cross-section of an embryonic day (E) 17 chick cerebellum. dI2 synapses (magenta), synaptotagmin (cyan). The arrowhead points to the midline, the yellow dash lines encircle the central cerebellar nuclei, and the area confined in the white square is enlarged in (A’). (B) Density plot of dI2 and precerebellar neurons at the crural plexus segments (N = 551 and N = 652 cells, respectively; N = 2 embryos). (C) Density plot of dI2 synapses and precerebellar neurons at the crural plexus segments (N = 2543 synapses with density values of 25–80% and N = 652 cells with density values of 10–90%, respectively). (D) Laminar distribution of precerebellar neurons, dI2 neurons, and dI2 synapses in the different lamina at the crural level.
Figure 2—figure supplement 1—source data 1. Localization of precerebellar neurons and dI2 synapses at the crural level.
The X/Y coordinates of precerebellar neurons (Figure 1—figure supplement 1B) and dI2 synapses (Figure 2—figure supplement 1C) at the crural level.