Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Trypanosoma brucei brucei) | ‘SIF’ cell line, EATRO 1125 Antat1.1(GFP:PAD1UTR) | The GFP:PAD1UTR reporter was verified in: Zimmermann et al., 2017 |
DOI:10.1371/journal.ppat.1006324 | Transfected with: p4231 (containing an NLS-GFP reporter fused to the 3’ UTR of the PAD1 gene, which inserts into the β-tubulin locus; provided by Mark Carrington) |
| Cell line (Trypanosoma brucei brucei) | ‘ES’ cell line, Antat1.1 13–90 (GFP:PAD1UTR) | This cell line was verified in: Zimmermann et al., 2017 | DOI:10.1371/journal.ppat.1006324 | Transfected with: pLew13 (Addgene plasmid 24007, G. Cross); pLew90 (Addgene plasmid 24008, G. Cross); p4231 (containing an NLS-GFP reporter fused to the 3’ UTR of the PAD1 gene, which inserts into the β-tubulin locus; provided by Mark Carrington) |
| Cell line (Trypanosoma brucei brucei) | ‘SIF’ cell line, EATRO 1125 Antat1.1(GFP:PAD1UTR) with an EP1:YFP fusion protein | The GFP:PAD1UTR reporter was verified in: Zimmermann et al., 2017 The EP1:YFP reporter was verified in: Engstler and Boshart, 2004 |
DOI:10.1371/journal.ppat.1006324
DOI: 10.1101/gad.323404 |
Transfected with: p4231 (containing an NLS-GFP reporter fused to the 3’ UTR of the PAD1 gene, which inserts into the β-tubulin locus; provided by Mark Carrington); pGaprone(ble)_EPGFG_rev GPY PARPYFP |
| Cell line (Trypanosoma brucei brucei) | Monomorphic cell line,T. brucei 427 MITat 1.2 13–90 | This cell line was verified in: Wirtz et al., 1999 | DOI: 10.1016/s0166-6851(99)00002-x | |
| Antibody | Anti-PAD1 (Trypanosoma brucei brucei) (Rabbit Polyclonal) |
This antibody was generated and verified in: Dean et al., 2009 | DOI: 10.1038/nature07997 | IF (1:100) |
| Antibody | Anti-EP1 (Trypanosoma brucei brucei) (Mouse monoclonal) |
CEDARLANE | Product code: CLP001A | IF (1:500) |
| Chemical compound, drug | Methylcellulose | Sigma Aldrich | Product code: 94378 | Methocel A4M |
| Software, algorithm | R | R core team | Package ‘mgcv’ | |
| Software, algorithm | ImageJ | NIH | DOI: 10.1038/nmeth.2089 |