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. 2021 Sep 10;59(4):82. doi: 10.3892/ijo.2021.5262

Figure 2.

Figure 2

Downregulation of ASIC1a promotes glioma cell proliferation, while overexpression of ASIC1a inhibits its growth. (A) A172 cells were transfected with shASIC1a (left), treated with ASIC1a selective inhibitor PcTx1 (right), and transfected with ASIC1a-GFP (middle) for 24 to 96 h followed by an MTT assay. (B) Transfection efficiency of A172 cells was revealed by the expression of GFP marker. (C) U87MG cells were transfected with shASIC1a (left), treated with ASIC1a selective inhibitor PcTx1 (right), and transfected with ASIC1a-GFP (middle) for 24 to 96 h followed by an MTT assay. (D) Transfection efficiency of U87MG cells was revealed by the expression of GFP marker. (E) PDX-L12 cells were transfected with shASIC1a (left), treated with ASIC1a selective inhibitor PcTx1 (right), and transfected with ASIC1a-GFP (middle) for 24 to 96 h followed by an MTT assay. (F) Transfection efficiency of PDX-L12 cells was revealed by the expression of GFP marker. *P<0.05. ASIC1a, acid-sensing ion channel 1a; sh, short hairpin; PcTx1, psalmotoxin; GFP, green fluorescent protein.