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. 2021 Sep 7;97(10):e975–e987. doi: 10.1212/WNL.0000000000012463

Figure 7. Patient Immunoglobulin (Ig) Inhibited Agrin-Enhanced Low-Density Lipoprotein Receptor-Related Protein 4 (LRP4)–Muscle-Specific Tyrosine Kinase (MuSK) Interaction.

Figure 7

(A) Increased LRP4-ECD-MuSK interaction by agrin. Condition medium of HEK293 cells transfected with LRP4-ECD-Myc was incubated with Flag-MuSK-ECD IgG beads in the presence or absence of agrin LG3. LRP4-ECD-Myc in the precipitates with Flag-MuSK-ECD-IgG beads was revealed by Western blotting. (B) Quantification of data in A. (C) Inhibition of agrin-enhanced LRP4–MuSK interaction by patient Ig. (D) Quantitative analysis of data in C. (E) Little effect of patient Ig on basal LRP4–MuSK interaction. (F) Quantitative data in E. n = 3, *p < 0.05, **p < 0.01.