Cisplatin-resistant cells and serum-free cultured cells from cervical cancer cell lines exhibit stem-like properties. (a–f) Detection of the stem cell markers NANOG, OCT4, CD44, ALDH1, CD133, and SOX2 in HeLa cells cultured in a serum-free medium by qRT-PCR. (g–l) Detection of the stem cell markers NANOG, OCT4, CD44, ALDH1, CD133, and SOX2 in cisplatin-resistant HeLa cells by qRT-PCR. (m) Western blot analysis of the stem cell markers NANOG, OCT4, CD133, and SOX2 in cisplatin-resistant HeLa cells. (n) Statistic analysis relative destiny of (m). ∗P values represent significant difference based on Student's t-test from three replicates (∗P, ∗∗P < 0.01).