Table 8.
Responses of soleus (Sol) muscle proteins involved in blood coagulation, fibrinolysis, innate immune response, and complement activation to microgravity (µ-g) or artificial 1-g (A1-g) exposure and/or fructo-oligosaccharide (FOS) ingestion.
| Gene symbol (accession) | µ-g & FOS (−)/A1-g & FOS (−) | µ-g & FOS (+)/A1-g & FOS (−) | A1-g & FOS (+)/A1-g & FOS (−) |
|---|---|---|---|
| Blood coagulation & fibrinolysis | |||
| Apoh (Q01339) | * FC = 4.97, P = 1.4E−4 | * FC = 6.37, P = 1.0E−4 | FC = 0.74, P = 8.3E−2 |
| F12 (Q80YC5) | FC = 2.09, P = 1.1E−2 | * FC = 3.67, P = 6.8E−4 | FC = 0.79, P = 3.0E−1 |
| Fga (E9PV24) | * FC = 3.46, P = 9.0E−3 | * FC = 3.49, P = 8.9E−3 | FC = 0.68, P = 3.6E−1 |
| Fgb (Q8K0E8) | * FC = 3.36, P = 2.8E−3 | * FC = 3.45, P = 2.2E−3 | FC = 1.02, P = 8.4E−1 |
| Fgg (Q8VCM7) | FC = 3.81, P = 1.1E−2 | * FC = 4.08, P = 8.6E−3 | FC = 0.80, P = 6.3E−1 |
| Hspb1 (P14602) | * FC = 1.59, P = 1.3E−3 | * FC = 1.80, P = 2.7E−3 | FC = 1.13, P = 1.1E−1 |
| Hrg (Q9ESB3) | * FC = 1.95, P = 7.6E−3 | FC = 2.89, P = 1.1E−2 | FC = 1.02, P = 8.2E−1 |
| Kng1 (O08677) | * FC = 3.38, P = 3.3E−4 | * FC = 4.72, P = 1.4E−3 | FC = 0.83, P = 2.9E−1 |
| Plg (P20918) | * FC = 6.06, P = 2.5E−4 | * FC = 8.12, P = 3.5E−4 | FC = 0.99, P = 9.6E−1 |
| Serpinc1 (P32261) | FC = 2.49, P = 1.1E−2 | * FC = 2.46, P = 5.1E−3 | FC = 0.77, P = 2.9E−1 |
| Serpind1 (P49182) | * FC = 5.84, P = 2.6E−4 | * FC = 7.81, P = 3.2E−4 | FC = 0.69, P = 1.2E−1 |
| Serpinf2 (Q61247) | * FC = 3.07, P = 1.3E−3 | * FC = 3.64, P = 3.8E−3 | FC = 0.89, P = 5.0E−1 |
| Serping1 (P97290) | * FC = 2.55, P = 6.0E−3 | * FC = 3.72, P = 2.6E−3 | FC = 0.74, P = 3.7E−1 |
| Innate immune response & complement activation | |||
| Ahsg (P29699) | * FC = 5.24, P = 1.5E−4 | * FC = 7.18, P = 4.4E−4 | FC = 0.80, P = 1.9E−1 |
| Agt (P11859) | * FC = 8.79, P = 3.4E−4 | * FC = 14.66, P = 1.3E−4 | FC = 0.94, P = 8.3E−1 |
| Anxa1 (P10107) | * FC = 2.78, P = 6.7E−4 | * FC = 2.50, P = 6.4E−3 | FC = 0.90, P = 4.2E−1 |
| Anxa11 (P97384) | FC = 1.13, P = 3.9E−2 | * FC = 1.26, P = 2.7E−3 | FC = 1.04, P = 3.1E−1 |
| C3 (P01027) | * FC = 4.48, P = 4.8E−4 | * FC = 5.31, P = 7.5E−4 | FC = 1.02, P = 9.0E−1 |
| C4b (P01029) | * FC = 3.69, P = 3.1E−4 | * FC = 5.06, P = 2.6E−4 | FC = 0.72, P = 1.1E−1 |
| C8g (Q8VCG4) | * FC = 3.60, P = 4.9E−4 | * FC = 3.95, P = 3.8E−3 | FC = 0.81, P = 4.1E−1 |
| Cfh (P06909) | * FC = 3.04, P = 2.5E−3 | * FC = 4.46, P = 1.3E−3 | FC = 1.07, P = 9.7E−1 |
| Cfi (Q61129) | * FC = 3.27, P = 1.2E−3 | * FC = 4.54, P = 2.5E−3 | FC = 0.82, P = 3.6E−1 |
| Cfb (P04186) | * FC = 1.98, P = 2.8E−3 | * FC = 2.22, P = 2.8E−3 | FC = 0.97, P = 8.1E−1 |
| Ighg1 (P01868) | FC = 5.87, P = 1.7E−2 | * FC = 6.29, P = 6.1E−4 | FC = 2.33, P = 1.7E−1 |
The protein abundance profiles in Sol muscles of the groups exposed to µ-g with and without FOS ingestion and the group exposed to A1-g with FOS ingestion were individually compared with that of the group exposed to A1-g without FOS ingestion. The results for the proteins, which were categorized as the related factors to blood coagulation fibrinolysis, innate immune response, and/or complement activation by DAVID Bioinformatics Resources 6.8 and significantly (p < 0.01) affected by µ-g exposure and/or FOS ingestion, are indicated. The fold change (FC) and p value (P) of each protein are depicted. *: the significant high- or low-abundance proteins.