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. 2021 Sep 17;12:5522. doi: 10.1038/s41467-021-25803-0

Fig. 6. The IL-15-AKT pathway is impaired in METTL3-deficient NK cells.

Fig. 6

a, b Representative histograms (a) and MFI (b) of CD122 and CD132 expression in splenic NK cells from WT (n = 6 for CD122, n = 4 for CD132) and cKO (n = 3 for CD122, n = 4 for CD132) mice. c, e, f Immunoblotting of indicated molecules in splenic NK cells upon stimulation with rmIL-15 (50 ng/mL) for 1 h. The NK cells were sorted from WT and cKO mice that received hydrodynamic injection of the pLIVE-IL-15 plasmid or pLIVE control plasmid 1–2 months previously. d Representative histogram (left) and MFI (right) of p-AKT (S473) expression in splenic NK cells upon stimulation with rmIL-15 (50 ng/mL) for 1 h (n = 4 for WT group; n = 6 for cKO group). g, h Splenic NK cells from WT or cKO mice were cultured with rmIL-15 overnight (10 ng/mL), and then the indicated compounds were added into each well at the indicated times. g The oxygen consumption rate was measured for NK cells. h Statistical graphs showing the maximal respiration and spare respiratory capacity of NK cells (n = 7 for WT group; n = 5 for cKO group). Each symbol represents an individual mouse (b, d) or an individual well of a cell culture plate (h). Data are the mean ± SEM (ns, not significant; unpaired two-tailed t test (b, d, h)). Source data are provided as a Source Data file. Data represent at least two independent experiments (a–h).