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. 2021 Sep 17;4:1093. doi: 10.1038/s42003-021-02625-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic diagram of Pib2(304-533, Δ356-384) was used for DSC measurements. b Pib2(304-533, Δ356-384) interacts with TORC1 in response to glutamine. In vitro pull-down assays were performed (as in Fig. 1c) using GST-Pib2(304-533, Δ356-384) and yeast cell lysates prepared from Kog1-myc-expressing cells (TS270) with or without the indicated concentrations of L-glutamine. The line graph shows Kog1-myc bound to GST-Pib2(304-533, Δ356-384) normalized to sample without glutamine. The bar graph shows GST-Pib2-bound FLAG-Tor1 normalized to the samples without glutamine. Error bars represent the standard deviation (n = 3 independent experiments). c, d L-glutamine enhanced the ΔH of Pib2 heat-induced unfolding. c Thioredoxin (Trx)-tagged Pib2(304-533, Δ356-384) was subjected to DSC measurements with or without the indicated amino acids (20 mM). Purified samples were analysed by SDS-PAGE followed by CBB staining. d Trx-tagged Pib2(304-533, R341A, Δ356-384) was subjected to DSC measurements as in (c). e L-glutamine did not affect Trx, as determined by DSC measurements. Trx was subjected to DSC measurements as in (c).