Table 2.
Plasmids used in this study.
| Plasmids | Description | Reference or source |
|---|---|---|
| pRS416 | Yeast shuttle vector | 26 |
| p416ADH | Yeast shuttle vector | 25 |
| p425ADH | Yeast shuttle vector | 25 |
| pET48b(+) | Trx-His tagged protein expression vector | Merck Millipore |
| pET49b(+) | GST-tagged protein expression vector | Merck Millipore |
| pET50b(+) | His-Nus-His tagged protein expression vector | Merck Millipore |
| pTBK1 | Intein-CBD tagged protein expression vector | New England Biolabs |
| pMH330 | p416ADH-PIB2 | This study |
| pMH334 | p416ADH-pib2 (Δ1-115) | This study |
| pMH342 | pRS416-PIB2 | This study |
| pMH410 | p416ADH-pib2 (Δ1-303) | This study |
| pMH417 | pET49b(+)-pib2 (304-635) | This study |
| pMH422 | pET49b(+)-pib2 (304-620) | This study |
| pMH426 | pET49b(+)-pib2 (440-635) | This study |
| pMH433 | pRS415-PIB2 | This study |
| pMH497 | pRS416-pib2(P337A) | This study |
| pMH498 | pRS416-pib2(R341A) | This study |
| pMH499 | pRS416-pib2(L340A) | This study |
| pMH508 | Same as pMH417 except PIB2(V626A) | This study |
| pMH510 | p416ADH-pib2(P337A) | This study |
| pMH512 | p416ADH-pib2(L340A) | This study |
| pMH514 | p416ADH-pib2(R341A) | This study |
| pMH553 | Same as pMH417 except PIB2(P627S) | This study |
| pMH554 | Same as pMH417 except PIB2(A628T) | This study |
| pMH555 | Same as pMH417 except PIB2(N629S) | This study |
| pMH568 | Same as pMH417 except (R341A, V626A) | This study |
| pMH606 | pET48b(+)-pib2(304-533, Δ356-384) | This study |
| pMH610 | pET50b(+)-pib2(304-533, Δ356-384) | This study |
| pMH649 | pTBK1-Trx-His-pib2(304-635) | This study |
| pKY11-1 | Same as pMH334 except PIB2(V626A) | This study |
| pKY14-1 | Same as pMH334 except PIB2(P627S) | This study |
| pKY24-1 | Same as pMH417 except PIB2(A628T) | This study |
| pKY21 | Same as pMH334 except PIB2(N629S) | This study |
| pKY95 | Same as pMH342 except pib2(Δ356-384) | This study |