Fig. 2. The phosphorylation of glycerol by imidazole phosphate in paste conditions.

a The phosphorylation reaction of glycerol by calcium imidazole phosphate in paste conditions after drying to a paste using the second method. b Representative ³¹P-NMR spectra over time for the reaction of 0.13 mmol calcium imidazole phosphate and 0.65 mmol glycerol in paste conditions at 50 °C. The paste was prepared by leaving 2.0 mL of a 65 mM calcium imidazole phosphate and 325 mM glycerol solution to dry for 48 h at 22 °C. The ³¹P-NMR spectrum at t = 0 h was taken from the paste after the evaporation of water. During the drying stage diphosphoimidazole formed from the reaction of imidazole phosphate with itself. c Yields of phosphate-containing compounds over time for the reaction in a and b.