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. 2021 Sep 17;12:5509. doi: 10.1038/s41467-021-25788-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a CAD drawing and (b) microscope image of membrane valve-based serial diluter. c Normalized fluorescein concentration as a function of time for all ten output channels (outputs 1–10) and the input concentration (output 0). d Steady state normalized fluorescein concentration at each output channel for three repeated tests. e Microscope image of 10-stage twofold serial diluter made with squeeze valve-based 1:1 mixer modules.