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. 2021 Sep 17;11:18581. doi: 10.1038/s41598-021-97972-3

Figure 1.

Figure 1

Both Pin1 knockdown and treatment with Pin1 inhibitors significantly reduced SARS-CoV-2 proliferation in VeroE6/TMPRSS2 cells. (A) VeroE6/TMPRSS2 cells were transfected with either Pin1 siRNA or negative siRNA, and after three days the cells were infected with SARS-CoV-2 at an input MOI of 0.01 followed by incubation for 24 h (n = 3–4). Cell lysates were processed for Western blotting with SARS-CoV-2 N, Pin1 and actin antibodies, and the bands are shown (Duplicate in the figure). The density of the N protein bands was quantitated, and relative density is shown in the graph with Negative siRNA as 1.0. ***P < 0.001, Mann–Whitney U test, compared with Negative siRNA. (B) The proliferation of SARS-CoV-2 in VeroE6/TMPRSS2 cells pretreated with 5 or 10 µM of each Pin1 inhibitor or 0 µM (DMSO). The cells were pretreated with a drug 2 h before infection and infected with SARS-CoV-2 at an MOI of 10, followed by incubation for 8 h in the presence of the drug. Cell lysates were processed for Western blotting using SARS-CoV-2 N, Pin1, and actin antibodies. Full-length blots are presented in Supplementary Fig. 3.