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. 2021 Sep 17;4:1098. doi: 10.1038/s42003-021-02619-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The transcript encoding the main SCRIB protein contains additional unidentified sORFs (uSCRIB and oSCRIB). The oSCRIB-coding region overlapped the downstream out-of-frame SCRIB gene in the human genome. Translational start codons (AUGs) of oSCRIB and SCRIB and the surrounding sequences in humans are shown. The most prevalent sequence (GGACU) for the reversible epitranscriptomic m⁶A modification and the resultant m⁶A-dependent start codon selection^42,43,58,73 are shown in the box. b Survey of publicly available Ribo-Seq data. The aggregated profiles of initiating and elongating ribosomes were obtained from GWIPS-viz⁷⁴ (https://gwips.ucc.ie). c Translation products of oSCRIB in humans (Homo sapiens), chimpanzees (Pan troglodytes), cattle (Bos taurus), mice (Mus musculus), and rats (Rattus norvegicus). The protein sequences were aligned and colored using the GenomeNet ClustalW 2.1 and EMBnet BoxShade 3.21 servers (https://www.genome.jp/tools-bin/clustalw and https://embnet.vital-it.ch/software/BOX_form.html). Red boxes indicate the amino acid sequences of tryptic peptides detected in LC–MS/MS analyses as shown in Figs. 3 and 4.