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. 2021 Sep 17;11:18547. doi: 10.1038/s41598-021-97532-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The Spearman rank correlation coefficient between the MO values and the estimated hemozoin content, $H Z = 1 \cdot R i + α \cdot T$ , as the function of $α$ . The red curve shows the correlation coefficient calculated for the subset of samples in which the proportion of schizonts and gametocytes was below 3% (n = 19). The black curve shows the correlation coefficient for the full sample set (n = 35). (B) The MO values as the function of parasite density (bottom x-axis, blue circles) and as the function of HZ (top x-axis, red diamonds).The latter was calculated using the value $α = 4.5$ (determined from the red curve in panel A), and also including the hemozoin contributions from schizonts and gametocytes, as described in the main text. The Spearman rank correlation coefficients for the two cases are also indicated.