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. 2021 Sep 17;11:18534. doi: 10.1038/s41598-021-97753-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometric analysis of CD14 selected monocytes used for dendritic cells differentiation. Flow cytometry dot plot analysis (A) Exclusion of doublets, gating on FSC-Area vs FSC-High; (B) sequential gate on cells expressing CD45; (C) and a final gate displaying 99% purity, almost all cells are identified as monocytes by the co-expression of CD14+ and CD33+.