FIG. 2.

Appearance of CWP1 during encystment. Culture supernatant fluids were sampled at the time points listed and assayed by immunoblotting and ELISA. (A) Immunoblot analysis of culture supernatant fluids. The MAb from the TechLab test was used as the detecting antibody. The 26-kDa band initially was detected at 18 h, and the 22-kDa band initially was detected at 42 h. (B) Analysis of culture supernatant fluids by the TechLab test and by the Alexon test. CWP1 initially was detected by both ELISAs at 10 h. CWP1 was not detected by ELISA or immunoblotting at any time in cultures of synchronized trophozoites.