Table 2.

Linearity and sensitivity of SOD1 and RPP30 duplex one-step RT-ddPCR

RNA input (ng)	Average measured SOD1 copies/μL	%CV	Average measured RPP30 copies/μL	%CV	GEX (SOD1:RPP30)	Average GEX (SOD1:RPP30)	%CV
25.0	6,287	0.9	1,093	2.3	5.75	5.64	2.1
12.5	3,063	2.4	539	3.1	5.69
6.25	1,504	1.2	261	2.1	5.76
3.125	753	1.5	131	3.5	5.76
1.563	369	2.5	64	2.9	5.76
0.781	183	2.8	32	7.3	5.67
0.391	89.9	2.1	16.2	5.1	5.55
0.195	43.0	3.9	7.7	10.5	5.56
0.098	19.4	10.3	3.6	11.7	5.44
0.049	10.3	1.7	1.9	13.5	5.52