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Accuracy and precision of the one-step RT-ddPCR method in quantifying the potency of a gene-repression vector (linearity)
| Expected % relative potency | Measured % relative potency | Average measured % relative potency | % Recovery |
|---|---|---|---|
| 150 | 162 | 164.0 | 109.3 |
| 166 | |||
| 125 | 132 | 130.5 | 104.4 |
| 129 | |||
| 75 | 72.9 | 72.8 | 97.0 |
| 72.6 | |||
| 50 | 48.9 | 49.5 | 99.0 |
| 50.1 | |||
| Accuracy (average % recovery) | 98.0 | ||
| Precision (%CV) | 1.4 |
