Figure 1.
Role of RGO-SMEDDS on hepatoma carcinoma cell growth in vitro
(A) Measurement of cell proliferation for 0, 0.15, 0.30, 0.60, and 1.20 μM RGO-SMEDDS-treated LO2, Huh7, HepG2, and Hep3B cells by CCK-8 assay. (B) IC50 of RGO-SMEDDS against HepG2 and Huh7 cells. (C) Effects of RGO-SMEDDS on the colony formation of HepG2 and Huh7 cells. (D) Cell cycle distribution of RGO-SMEDDS treated at different concentrations of Huh7 and HepG2 cells determined by flow cytometry. (E) Flow cytometry was used to detect apoptosis induced by RGO-SMEDDS. Values are shown as mean ± standard error from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.