Figure 6.

RGO-SMEDDS restrains stemness of HCC through the EGFR/AKT signaling pathway

(A) Representative IHC staining results of EGFR and p-EGFR in xenografts. Scale bars, 50 μm. (B) The effect of RGO-SMEDDS on EGFR signaling pathway in HepG2 and Huh7 cells was detected by WB assay. (C and D) The mRNA and protein expression level of stemness markers in RGO-SMEDDS-treated or untreated HepG2 and Huh7 cells determined by WB and qRT-PCR assay under the influence of Akt activator SC79. (E and F) Effects of RGO-SMEDDS and Akt activator SC79 alone or in combination on sphere-forming and clone-forming ability of cells. Scale bars, 50 μm. Values are shown as mean ± standard error from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.