Table 1.
List of primers used in this study
| PCR | Primer | Sequence (5′−3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| qRT- PCR | IL2F | TACAAGAACCCGAAACTGACTCG | 223 | 60 |
| IL2R | ACATGAAGGTAGTCTCACTGCC | |||
| IL4F | CCAACTGCTTCCCCCTCTG | 150 | 60 | |
| IL4R | TCTGTTACGGTCAACTCGGTG | |||
| IL10F | GACTTTAAGGGTTACCTGGGTTG | 112 | 60 | |
| IL10R | TCACATGCGCCTTGATGTCTG | |||
| IL13F | GAAGGCTCCGCTCTGCAAT | 75 | 60 | |
| IL13R | TCCAGGGCTGCACAGTACA | |||
| M-CSFF | TGGCGAGCAGGAGTATCAC | 108 | 60 | |
| M-CSFR | AGGTCTCCATCTGACTGTCAAT | |||
| CD163F | TTTGTCAACTTGAGTCCCTTCAC | 127 | 60 | |
| CD163R | TCCCGCTACACTTGTTTTCAC | |||
| ARG1F | GTGGAAACTTGCATGGACAAC | 76 | 60 | |
| ARG1R | AATCCTGGCACATCGGGAATC | |||
| VEGFAF | AGGGCAGAATCATCACGAAGT | 75 | 60 | |
| VEGFAR | AGGGTCTCGATTGGATGGCA | |||
| VEGFRF | GGCCCAATAATCAGAGTGGCA | 109 | 60 | |
| VEGFRR | CCAGTGTCATTTCCGATCACTTT | |||
| SOX2F | GCCGAGTGGAAACTTTTGTCG | 155 | 60 | |
| SOX2R | GGCAGCGTGTACTTATCCTTCT | |||
| NANOGF | TTTGTGGGCCTGAAGAAAACT | 116 | 60 | |
| NANOGR | AGGGCTGTCCTGAATAAGCAG | |||
| CD133F | AGTCGGAAACTGGCAGATAGC | 99 | 60 | |
| CD133R | GGTAGTGTTGTACTGGGCCAAT | |||
| OCT3/4F | CTGGGTTGATCCTCGGACCT | 243 | 60 | |
| OCT3/4R | CCATCGGAGTTGCTCTCCA | |||
| c-MycF | GGCTCCTGGCAAAAGGTCA | 119 | 60 | |
| c-MycR | CTGCGTAGTTGTGCTGATGT | |||
| β-actinF | GTCTTCCCCTCCATCGTG | 113 | 60 | |
| β-actinR | AGGGTGAGGATGCCTCTCTT |