ESC basal media

Reagent	Final concentration	Amount
Advanced DMEM/F12	1×	440 mL
FBS – ES Cell Qualified (not heat inactivated).	10% (v/v)	50 mL
Penicillin-Streptomycin	1% (v/v)	5 mL
2-mercaptoethanol	50 μM	454 μL
Glutamax	2 mM	5 mL
Mouse Leukemia Inhibitory Factor (LIF)	1 × 103 U/mL	50 μL
Total	n/a	500 mL

Filter sterile with a 0.22 μm filter. Store up to 4 weeks at 4°C.