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. 2021 Sep 15;2(3):100813. doi: 10.1016/j.xpro.2021.100813

HEK293T/LSL/MEF media

Reagent Final concentration Amount
Dulbeco’s Modified Eagle Medium 1× 440 mL
FBS 10% (v/v) 50 mL
Glutamax 2 mM 5 mL
Penicillin-Streptomycin 1% (v/v) 5 mL
Total n/a 500 mL

Store up to 4 weeks at 4°C