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. 2021 Aug 17;297(4):101084. doi: 10.1016/j.jbc.2021.101084

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

¹⁵N-HSQC of CjCBM5 interacting with (GlcNAc)₆. The figure shows an overlay of ¹⁵N-HSQC spectra for CjCBM5 in the presence of (GlcNAc)₆ at various concentrations (0.2, 1.0, 2.5, and 10 mM). The arrows indicate the direction of change in chemical shifts as a result of the titration of CjCBM5 with (GlcNAc)₆. Affected residues (W283, T284, Q285, Y296, and G297) are highlighted in green on the surface model of CjCBM5. Other surface-exposed aromatic residues for which no significant chemical shift perturbation was detected (Y265 and Y282) are shown in blue for illustration purposes. HSQC, heteronuclear single quantum coherence.