Table 3.

(R)-1,3-BDO and by-product yields in engineered C. necator strains. Cells were grown in 10 mL of 2 nitrogen-limiting minimal media supplemented with 2% sodium gluconate and 0.1% arabinose for 72 h.

Strain	Y1,3BDO (Cmol Cmol−1)	Y4H2B (Cmol Cmol−1)	YAcetate (Cmol Cmol−1)	YEthanol (Cmol Cmol−1)	YPyruvate (Cmol Cmol−1)
H16Δ1::54	0.008 ± 0.000	N.D.	0.022 ± 0.000	0.008 ± 0.000	0.345 ± 0.005
H16Δ1::54/Δ3::58	0.010 ± 0.001	N.D.	0.014 ± 0.002	0.013 ± 0.004	0.289 ± 0.023
H16Δ1::56	0.012 ± 0.001	N.D.	0.182 ± 0.010	0.108 ± 0.012	N.D.
H16Δ1::56/Δ3::58	0.017 ± 0.002	0.001 ± 0.000	0.136 ± 0.012	0.104 ± 0.003	0.016 ± 0.008
H16Δ1::56/Δ3::60	0.021 ± 0.001	0.001 ± 0.001	0.154 ± 0.012	0.110 ± 0.014	0.010 ± 0.005