Table 2.
Primary and Secondary Antibodies Used
| Antigen target | Host species | Dilution | Final antibody concentration | Conjugate | Manufacturer | Product number | RRID | Lot number |
|---|---|---|---|---|---|---|---|---|
| GFAP | Chicken | 1:1000 | 19.4 μg/mL | N/A | Abcam (Cambridge, MA) | ab4674 | AB_304558 | GR3368950-2 |
| MHC-II | Rat | 1:200 | 5 μg/mL | N/A | Novus Biologicals (Centennial, CO) | NBP2-21789 | AB_2828034 | 529699 |
| CD74 | Rabbit | 1:200 | 5 μg/mL | N/A | Bioss Antibodies (Woburn, MA) | BS-2518R | AB_10857560 | AG10193417 |
| P62 | Rabbit | 1:200 | 2.15 μg/mL | N/A | Abcam (Cambridge, MA) | ab109012 | AB_2810880 | GR3241806-2 |
| LC3B | Rabbit | 1:200 | 4.19 μg/mL | N/A | Abcam (Cambridge, MA) | ab192890 | AB_2827794 | GR3264793-8 |
| Anti-HA–Tag (C29F4) | Rabbit | 1:500 | 0.13 μg/mL | N/A | Cell Signaling Technology (Danvers, MA) | 3724 | AB_1549585 | B222922 / B272772 |
| HuC/HuD | Mouse | 1:200 | 1 μg/mL | Biotinylated | Thermo Fisher Scientific (Waltham, MA) | A-21272 | AB_2535822 | 2126799 |
| Anti-chicken IgY | Goat | 1:400 | 3.75 μg/mL | Dylight405 | Jackson ImmunoResearch (West Grove, PA) | 103-475-155 | AB_2337389 | 124380 |
| Anti-rat IgG | Goat | 1:400 | 3.75 μg/mL | AlexaFluor 594 | Jackson ImmunoResearch (West Grove, PA) | 112-585-003 | AB_2338372 | 105385 |
| Anti-rabbit IgG | Goat | 1:400 | 5 μg/mL | AlexaFluor 488 | Invitrogen (Waltham, MA) | A-11034 | AB_2576217 | 1141875 |
| Anti-mouse CD3 | Rat IgG2b, κ | 1:100 | 2 μg/mL | Brilliant Violet 421 | BioLegend (San Diego, CA) | 100228 | AB_2562553 | B311657 |
| CD45.2 | Mouse | 1:200 | 2.5 μg/mL | AlexaFluor 488 | BioLegend (San Diego, CA) | 109816 | AB_492868 | B280505 |
| CD4 | Rat | 1:200 | 2.5 μg/mL | FITC | BioLegend (San Diego, CA) | 100509 | AB_312712 | B269033 |
| CD8a | Rat | 1:200 | 1 μg/mL | APC/Cy7 | BioLegend (San Diego, CA) | 100713/100714 | AB_312752/AB_312753 | B283111/B260276 |
| Anti-mouse CD11b | Rat/IgG2b, κ | 1:200 | 1 μg/mL | PerCP-eFluor 710 | Thermo Fisher Scientific (Waltham, MA) | 46-0112-82 | AB_2866430 | 2336535 |
| Anti-mouse CD11c | Armenian hamster IgG | 1:100 | 2 μg/mL | PE | BioLegend (San Diego, CA) | 117307 | AB_313776 | B301182 |
| Anti-mouse CD19 | Rat IgG2a, κ | 1:100 | 2 μg/mL | APC | BioLegend (San Diego, CA) | 115512 | AB_313647 | B284257 |
| CD69 | Armenian hamster | 1:200 | 1 μg/mL | PE/Cy7 | BioLegend (San Diego, CA) | 104511/104512 | AB_493565/AB_493564 | B291440/B253212 |
| IFNγ | Rat | 1:100 | 2 μg/mL | PE | BioLegend (San Diego, CA) | 505808 | AB_315402 | B265789 |
| IL4 | Rat | 1:100 | 2 μg/mL | APC | BioLegend (San Diego, CA) | 504105 | AB_315319 | B270118 |
| IL17 | Rat | 1:20 | 10 μg/mL | Brilliant Violet 510 | BioLegend (San Diego, CA) | 506933 | AB_2562668 | B263584 |
| FoxP3 | Rat | 1:100 | 5 μg/mL | Pacific Blue | BioLegend (San Diego, CA) | 126410 | AB_2105047 | B274192 |
| TruStain FcX (anti-mouse CD16/32) | Rat IgG2a, λ | 1:100 | 5 μg/mL | N/A (Fc receptor block) | BioLegend (San Diego, CA) | 101320 | AB_1574975 | B305040 |
Cy7, Cyanine7; Fc, Fragment, crystallizable; FITC, fluorescein isothiocyanate; FoxP3, Forkhead Box P3; HA, hemagglutinin; HuC/HuD, Hu-antigen C/Hu-antigen D; PerCP, Peridinin-Chlorophyll-Protein; RRID, Research Resource Identifiers.