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. 2021 Aug 30;24(9):103065. doi: 10.1016/j.isci.2021.103065
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit monoclonal anti-Vimentin (1:100) Proteintech Cat#10366-1-AP; RRID: AB_2273020
Rabbit monoclonal anti-IGFBP1 (1:1000) Cell Signaling Technology Cat#31025; RRID: AB_2798998
Rabbit Polyclonal anti-PRL (1:1000) Affinity Biosciences Cat#DF6506; RRID: AB_2838468
Rabbit Polyclonal anti-TET3 (1:1000) Merck Millipore Cat#ABS463
Rabbit Polyclonal anti-Col1A1 (1:1000) Abcam Cat#ab34710; RRID: AB_731684
Rabbit Polyclonal anti-GAPDH (1:3000) Abcam Cat#ab9485; RRID: AB_307275
HRP-conjugated secondary antibody (1:5000) Abcam Cat#ab6721; RRID: AB_955447
Cy3-tagged secondary antibody (1:500) Boster Cat#BA1032; RRID: AB_2716305
Rabbit monoclonal anti-5hmC Abcam Cat#ab214728; RRID: AB_2797407
Rabbit Polyclonal anti-TET3 (ChIP) Sigma-Aldrich Cat#ABE290

Bacterial and virus strains

BJ5183 Unibio Cat#ST1039
XL1-Blue Unibio Cat#ST1054

Biological samples

Human endometrial tissues of proliferative-phase women and secretory-phase women Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University N/A
Human decidual tissues of EPL patients and normal early pregnancy women Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University N/A

Chemicals, peptides, and recombinant proteins

TRIzol Invitrogen Cat#15596-026
DMEM/F12 Procell Cat#PM150312
Fetal bovine serum (FBS) Procell Cat#164210-500
Trypsin Procell Cat#PB180225
Penicillin-Streptomycin Solution Procell Cat#PB180120
PBS Procell Cat#PB180327
Collagenase I Sigma Cat#C0130-100MG
TritonX-100 Beyotime Cat#ST795
PFA Beyotime Cat#P0099
Lipofectamine 3000 Thermo Fisher Scientific Cat#L3000-015
RIPA lysis buffer Beyotime Cat#P0013K
PMSF Beyotime Cat#ST505
DAPI Beyotime Cat#C1002
Oestradiol Targetmol Cat#T1048
Progesterone Targetmol Cat#T5040
cAMP Targetmol Cat#TC02745
DL2000 DNA Marker TIANGEN Cat#MD114-02

Critical commercial assays

SYBR Green PCR kit VAZYME Cat#Q111-02
Plasmid Midi kit Qiagen Cat#12143
CCK-8 assay kit Beyotime Cat#C0037
BCA Protein Assay Kit Beyotime Cat#P0010S
Dual Luciferase Reporter Kit Beyotime Cat#RG027
hMeDIP Kit Abcam Cat#ab117134

Experimental models: Organisms/strains

Mouse: C57BL/6 Vital River Animal Center,
Beijing, China
Cat#213

Oligonucleotides

Primers for miR-29a-3p: 5’-TGCGCTAGCACCATCTGAAATC-3’ and 5’-CCAGTGCAGGGTC CGAGGTATT-3’ This paper N/A
Primers for U6: 5’-CGCTTCGGCAGCACATATAC-3’ and 5’-AAATATGGAA CGCTTCACGA-3’ This paper N/A
Primers for TET1: 5’-CCTGCTCACGGCTCGGTTTTGATTG-3’ and 5’-G GGAGAGGCGGGTTGGATGATTACG-3’ This paper N/A
Primers for TET2: 5’-CCATTCCTGATACCATCAC CTCCCA-3’ and 5’-GGCTTACCCCGAAGTTACGTCTTTC-3’ This paper N/A
Primers for TET3: 5’-CCAAC GAGGAAATAGCGATTGACTG-3’ and 5’-CCCATTGTAGAGGTTATGCTGGTCC -3’ This paper N/A
Primers for Col1A1: 5’-GTTCCTCCCTGCTCTCCATC-3’ and 5’-GAGGTCCACAAAG CTGAACA-3’ This paper N/A
Primers for GAPDH: 5’-TCAAGAAGGTGGTGAAGCAGG-3’ and 5’-TCAA AGGTGGAGGAGTGGGT-3’ This paper N/A
MiR-29a agomir and antagomir GenePharma N/A
Control agomir and antagomir GenePharma N/A

Recombinant DNA

pAd5 vector Addgene Cat#122555
pGL3 reporter vector promega Cat#E1751
pRL-TK reporter promega Cat#E2241

Software and algorithms

SPSS 17.0 software SPSS Inc. N/A