Table 1.
Optimization of Ratio of FCS and blood for fixation
| FCS : Blood, (v/v) | Remarks |
|---|---|
| 1:0.1 | Fixed well, solution remained normal; cell recovery was 45%–64%. Nuclear morphology and integrity of WBCs, viz., neutrophils, lymphocytes and mix cells (monocytes, eosinophils and basophils), were intact/preserved well) |
| 1:0.15 | Fixed well, solution remained normal; cell recovery was 44%–62%. Nuclear morphology and integrity of WBCs, viz., neutrophils, lymphocytes and mix cells (monocytes, eosinophils and basophils), were intact/preserved well) |
| 1:0.2 | Fixed well, solution remained normal; cell recovery was 46%–61%. Nuclear morphology and integrity of WBCs, viz., neutrophils, lymphocytes and mix cells (monocytes, eosinophils and basophils), were intact/preserved well) |
| 1:0.25 | Fixed well, solution remained normal; cell recovery was 44%–67%. Nuclear morphology and integrity of WBCs, viz., neutrophils, lymphocytes and mix cells (monocytes, eosinophils and basophils), were intact/preserved well) |
| 1:0.30 | Fixed well, solution remained normal; cell recovery was 42%–68%. Nuclear morphology and integrity of WBCs, viz., neutrophils, lymphocytes and mix cells (monocytes, eosinophils and basophils), were intact/preserved well) |
| 1:0.35 | Fixed well, solution remained normal; cell recovery was 47%–66%. Nuclear morphology and integrity of WBCs, viz., neutrophils, lymphocytes and mix cells (monocytes, eosinophils and basophils), were intact/preserved well) |
| 1:0.40 | Fixed well, solution remained normal; cell recovery was 45%–65%. Nuclear morphology and integrity of WBCs, viz., neutrophils, lymphocytes and mix cells (monocytes, eosinophils and basophils), were intact/preserved well) |
| 1:0.45 | Fixed well, solution remained normal; cell recovery was 40%–61%. Nuclear morphology and integrity of WBCs, viz., neutrophils, lymphocytes and mix cells (monocytes, eosinophils and basophils), were intact/preserved well) |
| 1:0.5 | Fixed well, solution became viscous and cell recovery was poor (23%–49%).Pellet contained dense cell debris of RBCs and platelets that was difficult to remove) |
| 1:1 | Mixture solidified (cells could not be recovered) |
| 1:1.5 | Mixture solidified (cells could not be recovered) |
| 1:2 | Mixture solidified (cells could not be recovered) |