Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature

. 2021 Sep 15;2(4):100834. doi: 10.1016/j.xpro.2021.100834

Mixture 2: 0.8× Phosphate buffered saline (PBS) (7.0 mL/tube)

Reagent	NaCl	KCl	Na₂HPO₄	KH₂PO₄
Final concentration (mmol/L)	109.6	2.16	8.0	1.44
Final concentration (g/L)	0.64	0.16	1.136	0.192
(100 mL 0.8× PBS = 80 mL 1× PBS + 20 mL Distilled Water)