Mixture 3: Working FCS (7.5 mL/tube = 0.5 mL Stock FCS + 7 mL 0.8× PBS)

Reagent	Acetic acid	Methanol	Acetone	0.8× PBS
Final concentration	4.3%v/v	2.15%v/v	0.18%v/v	93.33%v/v