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. 2021 Sep 15;2(4):100834. doi: 10.1016/j.xpro.2021.100834
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins
Acetone (≥ 99.5% v/v) Sigma-Aldrich Cat# 179124
Glacial acetic acid (≥ 99%) Sigma-Aldrich Cat# A6283
Methanol (≥ 99.5% v/v) Sigma-Aldrich Cat# V800258
NaCl (≥ 99.5% (AT) Sigma-Aldrich Cat# S7653
KCl (≥ 99.0%) Sigma-Aldrich Cat# P9541
Na2HPO4 (≥ 99.0%) Sigma-Aldrich Cat# S9763
KH2PO4 (99.995%) Sigma-Aldrich Cat# 1.05108
Sodium citrate Sigma-Aldrich Cat# 6132-04-3
Triton X-100 (for molecular biology) Merck Cat# T8787
Tween-20 (for molecular biology) Merck Cat# P9416
DAPI with antifade (ProLong™ Gold Antifade Mountant with DAPI) Invitrogen Cat# P36962
Biological samples
Human, peripheral blood sample (from 5 volunteers; 3 males [age; 26, 34, and 39 years] and 2 females [age; 23 and 31 year]) Local healthy volunteers NA
Critical commercial assays
RBC lysis buffer BioLegend Cat# 420302
FISH probes (for chromosome pair one) MetaSystems Cat# D-0301-050-OR
FISH probes (for centromere of chromosome pair one) MetaSystems Cat# D-0801-050-OR
Software and algorithms
OriginLab 2018 (9.5) OriginLab https://www.originlab.com
Other
Falcon™ 15 mL Conical Centrifuge Tubes Fisher Scientific Cat# 14-959-53A
Deionized water Thomas Scientific Cat# C843U58
Doble frosted glass slides Borosil Microscope Slide Cat# 9100D01
Cover glass Borosil Microscope Cover glass Cat# 9115S02
Benchtop centrifuge Thermo Scientific™ Sorvall ST8R
Blood irradiator BRIT, India Blood Irradiator-2000
CO2 incubator Thermo Scientific™ Cat# 310TS
Swinging Bucket Clinical Rotor Thermo Scientific™ TX-100
Reagent Square Glass Bottles, 500 mL Borosil® GL80 PP CS/10
Reagent Square Glass Bottles, 500 mL Borosil® Borosil 1501021
Membrane filter, 0.22 μm pore size Millipore Cat# GSWP04700
Micropipette 20–200 μL Gilson, PIPETMAN LP200L FA10005M
Micropipette, 100–1000 μL Gilson, PIPETMAN L P1000L FA10006M
Fluorescence microscope (with blue and red filters) Leica TCS SP8 confocal
Type F, immersion oil for fluorescence Leica Cat# 11513859
Digital water bath with fine temperature control Equitron Cat#7505
Thermometer Star 49 Cat# B 34
ThermoBrite System Abbott Cat# 07J91-010
Freezer −20°C Samsung Cat# RT77KBTS
pH meter Hanna edge®
Forceps Tarsons Cat# 486000
Timer watch Fisherbrand Cat# 14–648-3
Glass Coplin Jar with glass lid Borosil Cat# SJ322
Rubber cement Fevibond NA
Humidified chamber (made in the laboratory) NA NA