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. 2021 Sep 15;2(4):100821. doi: 10.1016/j.xpro.2021.100821

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Imaging flow cytometry analysis of EV associated markers

Number of positive events (particles) in skim milk (SM) and infant milk formula (IMF) samples analyzed by imaging flow cytometry (IFCM). The events were gated for low side scatter channel (SSC) signals and no signals on brightfield, based on EV size and events positive for these gating was acquired as objects (+EVs) in low SSC. The number of positive CD9, CD63, CD81, ADAM10 and HLADR particles in GUC samples are presented in the lower panel. The IDEAS software was used to analyze the data, data presented as means and individual values ± SEM. A two-way ANOVA test was performed to compare differences in EVs in SM and IMF samples from each treatment groups; ∗indicates p<0.05. ∗∗indicates p<0.01 and ∗∗∗indicates p<0.001. Figure reprinted with permission from Mukhopadhya et al. (2021).