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. 2021 Sep 15;2(4):100821. doi: 10.1016/j.xpro.2021.100821

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Particle characterization and visualization of skim milk and infant milk formula EVs by nanoparticle tracking analysis and transmission electron microscopy

EV fractions generated post ultracentrifugation (GUC), and differential ultracentrifugation (DUC) were analyzed using a NanoSight NS300 analyzer (top panel). The EV enriched fractions were diluted accordingly, injected into the analyzer and the NTA 3.1.54 software was used to capture and analyze the EV size and particle concentration of the sample. Representative transmission electron microscope (TEM) images from GUC and DUC samples (bottom panel). Red arrows point towards vesicular structures in the sample. Scale bar=100 nm. Figure reprinted with permission from Mukhopadhya et al. (2021).