| Chemicals, peptides, and recombinant proteins |
|
| Acetic acid |
Sigma-Aldrich |
Cat# 338826 |
| Hydrochloric acid |
Thermo Fisher Scientific |
Cat# 1559100 |
| PBS (without calcium or magnesium, pH 7.2) |
Sigma Aldrich |
Cat# D8537 |
| Iodixanol solution OptiPrep™ (60% (w/v)) |
Sigma Aldrich |
Cat# D1556 |
|
| Biological samples |
|
| Skim milk/ defatted milk (< 1% fat) |
Local sources |
n/a |
| Infant milk formula powder |
Local sources |
n/a |
|
| Other |
|
| Optima XPN-100 Ultracentrifuge |
Beckman Coulter |
Cat# A94469 |
| Type 70.1 Ti Fixed-Angle Titanium Rotor |
Beckman Coulter |
Cat# 342184 (https://www.beckman.com/centrifuges/rotors/fixed-angle/342184) |
| SW Type 32 Ti swinging bucket rotor |
Beckman Coulter |
Cat# 369650 (https://www.beckman.com/centrifuges/rotors/swinging-bucket/369650) |
| 17 mL polyallomer tube |
Beckman Coulter |
Cat# 344061 |
| 38 mL open-top polypropylene tubes |
Beckman Coulter |
Cat# 326823 |
| pH meter |
Mettler Toledo, FiveEasy Standard pH Meter Line |
Cat# 30266627 |
| Sorvall ST 8 R centrifuge |
Thermo Fisher Scientific |
Cat# 75007205 |
| Pal-Ri Refractometer |
ATAGO |
Cat# 3850 |
| WhatmanTM Grade 1 filter paper (diameter 150 mm) |
GE Healthcare Life Sciences |
Cat# 1201 |
| 0.45-μm membrane syringe filters |
Thermo Fisher Scientific |
Cat# 15216869 |
| 0.22-μm membrane syringe filters |
Thermo Fisher Scientific |
Cat# 15206869 |
| Disposable needles, 18-gauge, 1.5in. long, w/polypropylene luer-fitting |
BD Microlance |
Cat# 304622 |
| Disposable syringe, 10 mL, luer-lock, sterilized |
VWR |
Cat# 613-2008 |