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. 2021 Sep 15;2(4):100821. doi: 10.1016/j.xpro.2021.100821
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

Acetic acid Sigma-Aldrich Cat# 338826
Hydrochloric acid Thermo Fisher Scientific Cat# 1559100
PBS (without calcium or magnesium, pH 7.2) Sigma Aldrich Cat# D8537
Iodixanol solution OptiPrep™ (60% (w/v)) Sigma Aldrich Cat# D1556

Biological samples

Skim milk/ defatted milk (< 1% fat) Local sources n/a
Infant milk formula powder Local sources n/a

Other

Optima XPN-100 Ultracentrifuge Beckman Coulter Cat# A94469
Type 70.1 Ti Fixed-Angle Titanium Rotor Beckman Coulter Cat# 342184 (https://www.beckman.com/centrifuges/rotors/fixed-angle/342184)
SW Type 32 Ti swinging bucket rotor Beckman Coulter Cat# 369650 (https://www.beckman.com/centrifuges/rotors/swinging-bucket/369650)
17 mL polyallomer tube Beckman Coulter Cat# 344061
38 mL open-top polypropylene tubes Beckman Coulter Cat# 326823
pH meter Mettler Toledo, FiveEasy Standard pH Meter Line Cat# 30266627
Sorvall ST 8 R centrifuge Thermo Fisher Scientific Cat# 75007205
Pal-Ri Refractometer ATAGO Cat# 3850
WhatmanTM Grade 1 filter paper (diameter 150 mm) GE Healthcare Life Sciences Cat# 1201
0.45-μm membrane syringe filters Thermo Fisher Scientific Cat# 15216869
0.22-μm membrane syringe filters Thermo Fisher Scientific Cat# 15206869
Disposable needles, 18-gauge, 1.5in. long, w/polypropylene luer-fitting BD Microlance Cat# 304622
Disposable syringe, 10 mL, luer-lock, sterilized VWR Cat# 613-2008