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Initial characterization of antibody-antigen interaction
Biotinylated antibodies (mAb1-3) are loaded on separate streptavidin (SA) sensors (I; loading), washed (II; baseline) and allowed to bind the antigen (III; association). The sensors (loaded with the antibody-antigen complex) are then dipped in the binding buffer (IV) to measure dissociation. Different mAbs may exhibit different levels of loading, which may affect subsequent Rmax.
