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Recommended plate layout for epitope binning
One mAb is used as capture antibody (in our assay this antibody is biotinylated: b-mAb1; column 2). A total of seven different antibodies can be measured at each run (represented by different colors in column 5) whereas an identical mAb (unlabeled mAb1) serves as control (well 5A). The antigen is used in a single concentration.
