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Representative assay run for epitope binning
Mab1 is used as capture antibody, loaded on the sensor, and also as second antibody in the control sample (black curve). Following antigen binding to mAb1, antibodies 1–8 are added. Only mAbs 4–6 bind the antigen in the presence of mAb1, as reflected by the wavelength shift. All other mAbs do not bind, giving a flat curve, similar to the control.
