Table 1.
Pooled bile acid profile by UPLC-MS/MS.
| WT | Cyp2c+/− | Cyp2c−/− | |
|---|---|---|---|
| ⍺-MCA | 18.9 ± 3.9 | 19.1 ± 3.9 | 0.79 ± 0.01b,d |
| β-MCA | 62.05 ± 11.7 | 38.2 ± 8.2 | 0.35 ± 0.01b,d |
| T⍺βMCA | 589.0 ± 139.5 | 456.0 ± 53.2 | 16.1 ± 3.2b,d |
| CDCA | 9.4 ± 1.4 | 17.0 ± 3.8 | 222.1 ± 77.9a,c |
| TCDCA | 32.9 ± 8.8 | 55.1 ± 11.2 | 1322.3 ± 208.4b,d |
| GCDCA | n.d | 0.27 ± 0.01 | 2.44 ± 0.7 |
| CA (12α –OH) | 360.4 ± 85.6 | 367.0 ± 89.2 | 170.0 ± 60.9 |
| TCA (12α –OH) | 1325.7 ± 368.4 | 1055.7 ± 200.1 | 976.0 ± 143.9 |
| GCA (12α –OH) | 3.9 ± 1.2 | 5.6 ± 2.7 | 3.1 ± 0.3 |
| DCA (12α –OH) | 20.2 ± 5.0 | 15.7 ± 4.4 | 7.4 ± 4.6 |
| TDCA (12α –OH) | 53.9 ± 23.5 | 32.7 ± 8.5 | 34.3 ± 11.0 |
| GDCA (12α –OH) | 0.25 ± 0.08 | 0.2 ± 0.04 | 0.2 ± 0.01 |
| LCA | n.d | n.d | n.d |
| TLCA | 0.3 ± 0.08 | 0.4 ± 0.1 | 11.0 ± 2.1b,d |
| UDCA | 4.6 ± 0.9 | 6.1 ± 0.6 | 30.0 ± 15.2 |
| TUDCA | 31.0 ± 9.0 | 27.5 ± 3.8 | 137.3 ± 37.33a,c |
| GUDCA | n.d | n.d | n.d |
| HDCA | 4.1 ± 0.2 | 4.5 ± 0.5 | n.d |
| Total BAs | 2513.6 ± 566.5 | 2096.1 ± 315.8 | 2922.2 ± 214.8 |
| Conjugated BAs | 2035.4 ± 532.6 | 1630.1 ± 253.9 | 2502.1 ± 236.3 |
| Non conjugated BAs | 478.2 ± 105.1 | 466.0 ± 105.5 | 420.1 ± 153.1 |
| Primary BAs | 2401.0 ± 545.1 | 2010.9 ± 302.2 | 2712.2 ± 193.8 |
| Secondary BAs | 112.6 ± 27.6 | 85.1 ± 15.6 | 210.0 ± 53.5 |
| Total 12⍺-OH BAs | 1762.9 ± 412.9 | 1474.0 ± 259.5 | 1190.2 ± 141.2 |
| Total non-12⍺-OH BAs | 750.7 ± 159.0 | 622.1 ± 73.0 | 1732.0 ± 211.7b,d |
Values are means ± SE in nmol/g tissue. α-MCA, α-muricholic acid; β-MCA, β-muricholic acid; TαβMCA, tauro-α- and tauro-β-muricholic acid; CDCA, chenodeoxycholic acid; TCDCA, taurochenodeoxycholic acid; GCDCA, glycochenodeoxycholic acid; CA, cholic acid; TCA, taurocholic acid; GCA, glycocholic acid; DCA, deoxycholic acid; TDCA, taurodeoxycholic acid; GDCA, glycodeoxycholic acid; LCA, lithocholic acid; TLCA, taurolithocholic acid; UDCA, ursodeoxycholic acid; TUDCA, tauroursodeoxycholic acid; GUDCA, glycoursodeoxycholic acid; HDCA, hyodeoxycholic acid; BA, bile acid; n.d, not detected. Species marked “(12α-OH)” are 12α-hydroxylated, unmarked are non-12α-hydroxylated. ap < 0.05, bp < 0.001 vs. WT control; cp < 0.05, dp < 0.001 vs. Cyp2c+/- measured by one-way ANOVA.