Table 2.
Example staining panel for functional analysis of lymphocytes after stimulation on a 5 laser BD LSRFortessa flow cytometer
| Laser | Detector | Fluorophore | Antigen | Dilution |
|---|---|---|---|---|
| 355 | 379/28 | BUV395 | CD8α | 1:400 |
| 355 | 450/50 | UV | Live/Dead | 1:1000 |
| 405 | 525/50 | BV510 | CD49a | 1:50 |
| 405 | 610/20 | SuperBright600 | CD4 | 1:700 |
| 405 | 670/30 | BV650 | CD8β | 1:1500 |
| 405 | 780/60 | BV785 | IFNγ | 1:50 |
| 488 | 530/30 | FITC | CD45.2 | 1:100 |
| 488 | 695/40 | PerCPCy5.5 | Granzyme B | 1:20 |
| 561 | 582/15 | PE | IL-10 | 1:100 |
| 561 | 610/20 | PECF594 | FoxP3 | 1:100 |
| 561 | 780/60 | PECy7 | CD69 | 1:50 |
| 640 | 670/30 | APC | CD1dtet | 1:150 |
| 640 | 730/45 | AF700 | TCRβ | 1:50 |
| 640 | 780/60 | APCeF780 | CD45.1 | 1:200 |