Table 3.
Example staining panel with options for phenotypic analysis of lymphocyte populations on a 5 laser BD LSRFortessa flow cytometer
| Laser | Detector | Fluorophore | Antigen | Dilution |
|---|---|---|---|---|
| 355 | 379/28 | BUV395 | CD8α | 1:500 |
| 355 | 450/50 | UV | Live/Dead | 1:1000 |
| 405 | 450/50 | BV421 | CX3CR1 or GATA3 or PD1 | 1:2000 or 1:20 or 1:50 |
| 405 | 525/50 | BV480 | CD8β | 1:1000 |
| 405 | 610/20 | BV605 | T-bet | 1:20 |
| 405 | 670/30 | BV650 | CXCR3 or LAG3 or RORγt | 1:100 or 1:50 or 1:20 |
| 405 | 710/50 | BV711 | CD69 or KLRG1 or CD49d | 1:20 or 1:200 or 1:20 |
| 405 | 780/60 | BV786 | CD4 | 1:1000 |
| 488 | 530/30 | FITC | CD45.2 | 1:100 |
| 488 | 695/40 | BB700 | CD49a | 1:100 |
| 561 | 582/15 | PE | CXCR6 or CD127 or TIM3 | 1:1000 or 1:100 or 1:300 |
| 561 | 610/20 | PECF594 | FoxP3 | 1:100 |
| 561 | 780/60 | PECy7 | CD69 or Ly6C or Eomes | 1:50 or 1:1000 or 1:70 |
| 640 | 670/30 | APC | CD1dtet | 1:200 |
| 640 | 730/45 | AF700 | TCRβ | 1:50 |
| 640 | 780/60 | APCeF780 | CD45.1 | 1:200 |