Heart digestion cocktail
| Reagent | Final concentration | Amount |
|---|---|---|
| Collagenase II (10,000 U/mL) | 400 U/mL | 200 μL |
| DNase I (2U/ μL) | 48 U/mL | 120 μL |
| DMEM | n/a | 4.68 mL |
| Total | n/a | 5 mL |
Prepare fresh immediately before use.
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