Alternative resources table

Reagent or resource	Alternative	Source	Identifier
Surgical scissors, forceps, and clamp forceps	Any similar instruments that enable dissection of the required tissue	Various	Various
DMEM	RPMI-1640 (ATCC modification)	Thermo Fisher	Cat#A1049101
Collagenase II	Collagenase II	Thermo Fisher	Cat#17101015
	Collagenase II	Stem Cell Technologies	Cat#07419
	Collagenase II	Merck	Cat#C2-22
DNase I	DNase I	Thermo Fisher	Cat#EN0521
	DNase I	Sigma-Aldrich	Cat#DN25
Red Blood Cell Lysis Solution	Red Blood Cell Lysis Solution	Tonbo	Cat#TNB-4300
	Red Blood Cell Lysis Solution	BioLegend	Cat#420301
42% Isotonic Percoll	Liver – 44% Isotonic Percoll
	Heart – 38%–42% Isotonic Percoll
Zombie UV fixable viability kit	Fixable Viability dye eFluor 455 UV	Thermo Fisher	Cat#65–0868
	Blue Live/Dead Fixable Dead Cell Stain	Thermo Fisher	Cat#L23105
	Fixable Viability Stain 450	BD Biosciences	Cat#562247
	GloCell Fixable Viability Dye UV450	Stem Cell Technologies	Cat#75008.1
FoxP3/Transcription factor staining buffer set	Transcription factor staining buffer set	Miltenyi	Cat#130-122-981
	Transcription factor buffer set	BD Biosciences	Cat#562574
	True-Nuclear Transcription Factor Buffer Set	BioLegend	Cat#424401
Cell Activation Cocktail	Leukocyte Activation Cocktail with BD GolgiPlug	BD Biosciences	Cat#550583
	PMA Ionomycin Brefeldin-A	Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich	Cat#P1585 Cat#I3909 Cat#B6542
Manual or automated cell counting	CountBright Absolute Counting Beads	Thermo Fisher	Cat#C36950
	Precision Count Beads	BioLegend	Cat#424902
Flowjo	Kaluza	Beckman Coulter	RRID:SCR_016182
	FCS Express	De Novo Software	RRID:SCR_016431
	CytoBank	N/A	RRID:SCR_014043

This protocol has been optimized for use with the resources and reagents listed in the key resources table. The table above is not an exhaustive list of available alternatives, though the suggested resources are expected to produce similar results. Any alternative resource or reagent requires validation before experimental use.