REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
α-FLAG (M2)	Millipore Sigma	Cat# A8592: RRID:AB_439702
α-McpA	Brun Lab	N/A
Goat Anti-Mouse IgG HRP conjugate	Millipore Sigma	Cat# AP130P, RRID:AB_91266
Bacterial strains
Caulobacter crescentus strains, see Table S1	This study, Poindexter, 1964, Sprecher et al.2017, Toh et al., 2008, Chepkwony et al., 2019 and Hardy et al., 2010	N/A
Hirschia baltica strains, see Table S1	This study, Chepkwony et al., 2019 and Schlesner et al., 1990	N/A
Chemicals, peptides, and recombinant proteins
Alexa Flour conjugated Maleimide C5 (AF488-mal)	ThermoFisher Scientific	Cat# A10254
Alexa Flour conjugated wheat germ agglutinin (AF594WGA and AF488WGA)	Molecular Probes	Cat# W11261, W11262
Alexa Flour conjugated conjugated Griffonia simplicifolia l (AF594GSL1)	Molecular Probes	Cat# L21416
EcoRV-HF endonuclease	New England Biolabs	Cat# RS3195S
NEBuilder® HiFi DNA Assembly master mix	New England Biolabs	Cat# E5520S
SuperSignal West Dura Substrate	Thermo Scientific	Cat# 34075
DifcoTM Marine Broth/Agar	BD	reference 2216/
Peptone Yeast Extract (PYE)	BD, Poindexter, 1964	Ref 211677, 212750,214010
Lysogeny broth (LB)	BD, Bertani G., 1951	Ref 211705, 214010, 214010
Kanamycin Sulfate	VWR	CAS# 25389-94-0
Experimental models: organisms/strains
E. coli α-select competent cells	Bioline	Cat# Bio-85025
E. coli BL21(DE3)	New England Biolabs	Cat# 69450
Oligonucleotides
Primers for gene deletion, complementations and overexpression of hfsH, see Table S2	This paper	N/A
Recombinant DNA
pET28a(+)	Novagen	Cat# 69865
pET28a hfsHCC	This study	N/A
pET28a hfsHHB	This study	N/A
pMR10	R. Roberts and C. Mohr	N/A
pMR10 with gene constructs, see Table S1	This Study	N/A
pNPTS139	M.R..K Alley	N/A
pNPTS139 with gene constructs, see Table S1	This study	N/A
Software and algorithms
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
MicrobeJ	Ducret et al., 2016	https://www.nature.com/articles/nmicrobiol201677
GraphPad Prism	GraphPad Software, LLC	https://www.Graphpad.com