Figure 2.
Fate mapping intratumoral hypoxia
(A and B) Lentiviral vectors used to generate the hypoxia fate mapping system. Fluorescent images (A) and flow cytometry analysis (B) of the MDA-MB-231 hypoxia fate mapping cells following exposure to 20% or 0.5% O2 for 5 days.
(C) Fluorescent images of the full cross section of an orthotopic tumor derived from MDA-MB-231 hypoxia fate-mapping cells.
(D) O2 measurements performed using a fixed-needle microprobe are displayed as a function of distance from the tumor core. Data are represented as mean ± SEM.
(E and F) Representative spheroid used to track migrating tumor-derived GFP+ or DsRed+ cells (E) and corresponding average speed and maximum displacement of individual cells (F) fit by Gaussian nonlinear regression.