Figure 3.

NK cells are a main source of XCL1 but not a critical one upon MCMV infection

(A and B) Analysis of mTFP1 expression in splenocytes of Wt (dark grey) and Xcl1-mTfp1^fl/fl (white) mice at steady state (A) or 40 h after infection (B). Cells were gated as follow: NK cells (TCRβ^- CD19^- NK1.1⁺), ILC1 (NK1.1⁺ TCRβ^- CD19^- CD127⁺), NKT cells (CD19^- CD1d⁺), CD8⁺ T (CD1d^- NK1.1^- TCRβ⁺ CD19^- CD8⁺), CD4⁺ T (CD1d^- NK1.1^- TCRβ⁺ CD19^- CD4⁺), γδ T cells (CD1d^- NK1.1^- CD3ε⁺ TCRβ^- TCRγδ⁺) and B cells (NK1.1^- TCRβ^- CD19⁺). One representative experiment of 4 independent ones with at least three mice per group is shown.

(C and D) Proportion of immune populations within mTFP1-positive cells at steady state (C) and 40 h after MCMV infection (D). Others: sum of all the other cell subsets not detailed in the pie charts. One representative experiment of 4 independent ones with at least three mice per group is shown.

(E) IFN-γ production by NK cells in Wt, Xcr1^−/−, Xcl1^fl/fl, and Nkp46^Cre;Xcl1^fl/fl mice 40 h after infection. One representative experiment of two independent ones with at least four mice per group is shown. A one-way ANOVA statistical analysis with a Tukey's multiple comparison test was applied. ∗, p < 0.05; ∗∗, p < 0.01, n.s., nonsignificant; ANOVA, analysis of variance.

See also Figure S3.